Chromosome conformation capture resolved near complete genome assembly of broomcorn millet
نویسندگان
چکیده
منابع مشابه
Hi-C: Genome-wide Chromosome Conformation Capture
The three-dimensional conformation of chromosomes in the nucleus is important for many cellular processes, including the regulation of gene expression, DNA replication, and chromatin structure [1]. Despite having the entire sequence of the genome, very little has been understood about three-dimensional chromosome conformation beyond the scale of the nucleosome. However, recent advances in molec...
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Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome co...
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Examples Determine interaction between a known promoter and enhancer Determine comprehensively higher-order chromosome structure in a defi ned region All genes and genomic elements associated with a known LCR All intraand interchromosomal associations Determine the role of specifi c transcription factors in the interaction between a known promoter and enhancer Map chromatin interaction network ...
متن کاملChromosome Conformation Capture (3C) (PROT05)
An alternative protocol (PROT31) for 3C has been adopted by the author's lab. Much of it is identical to this version. The major differences are in the amounts of DNA used at different steps. We reliably get the same results as with this version, but also a greater yield of 3C material. Yields from this version are ample for real-time PCR analysis, but greater yields are required if the 3C mate...
متن کاملThe statistical-mechanics of chromosome conformation capture
Since Jacob and Monod's characterization of the role of DNA elements in gene control, it has been recognized that the linear organization of genome structure is important for the regulation of gene transcription and hence the manifestation of phenotypes. Similarly, it has long been hypothesized that the spatial organization (in three dimensions evolving through time), as part of the epigenome, ...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-018-07876-6